Dr. Kamaleshwar Singh

Associate Professor, Environmental Genomics and Molecular Carcinogenesis

Graduate Advisor

 

1997-2002, Postdoctoral Training (Environmental Cancer Genomics & Molecular Carcinogenesis), Department of Environmental Health Sciences, University of Alabama at Birmingham, Birmingham, AL

1997, Doctor of Philosophy (Molecular Genetics), University of Delhi, India

1989, Master of Science (Genetics), L N Mithila University, Darbhanga, India

1985, Bachelor of Science (Biology), L N Mithila University, Darbhanga, India

 

Research Interests:

Dr. Singh’s research interests are molecular toxicology, environmental carcinogenesis, epigenetic reprogramming in kidney diseases and carcinogenesis. Dr. Singh has identified two novel genes associated with human breast and kidney cancer. His current research is focused on the role of environmental toxicants- and heavy metal-induced epigenetic reprogramming in human cancers and kidney fibrosis. Following are the topics of research being pursued in his research group:

  1. Role of oxidative stress in epigenetic reprogramming during arsenic and nicotine-induced carcinogenesis and kidney fibrosis
  2. Role of antioxidant and demethylating potentials of natural product green tea polyphenol in anticancer and antifibrotic signaling pathways
  3. Epigenetic mechanism of chemotherapeutic resistance development and potential of epigenetic-based therapeutics in resensitization of drug-resistant breast and kidney cancers

 

Current Research Funding: NIH (NIDDK)

 

Classes:

ENTX 6328 Molecular Methods in Toxicology

ENTX 6326 Principles of Toxicology

ENTX 6100 Advance Topics in Molecular Carcinogenesis

ENTX 6115 Environmental Epigenetics Seminar

 

Bio:

Dr. Singh received his PhD degree in Molecular Genetics from University of Delhi, India. He completed postdoctoral training from the University of Alabama at Birmingham.  In 2009, he joined the Department of Environmental Toxicology, and The Institute of Environmental and Human Health (TIEHH), Texas Tech University, Lubbock, Texas. He has published over 50 research papers in peer reviewed journals, book chapters and has presented his research in several research meetings. He is a member of American Association of Cancer Research (AACR), and Society of Toxicology (SOT). Dr. Singh serves as editorial board member for peer-reviewed journals (PLoS ONE, Bulletin of Environmental Contaminants and Toxicology). He has served as scientific review panel member for federal and private funding agencies as well as reviewer for many peer-reviewed journals.

 

Book Editor:

Molecular Toxicology Protocols (3rd Edition, 2020), Methods in Molecular Biology Series. Springer Science Publication. Editors: Phouthone Keohavong, Kamaleshwar P Singh, Weimin Gao.

 

 

Selected Publications:

  1. Acharya N, Singh KP (2021) Differential sensitivity of renal carcinoma cells to doxorubicin and epigenetic therapeutics depends on the genetic background. Molecular and Cellular Biochemistry 476:2365–2379
  2. Ponnusamy L, Mahalingaiah PK, and Singh KP (2020) Epigenetic reprogramming and potential application of epigenetic-modifying drugs in acquired chemotherapeutic resistance.  Advances in Clinical Chemistry 94:219-259.
  3. Chang YW, Singh KP (2019) Nicotine-induced oxidative stress contributes to EMT and stemness during neoplastic transformation through epigenetic modifications in human kidney epithelial cells. Toxicology Applied Pharmacology 374:65-76.
  4. Chang YW, Singh KP (2019) Arsenic-induced neoplastic transformation involves epithelial-mesenchymal transition and activation of the beta-catenin/c-Myc pathway in human kidney epithelial cells. Chemical Research in Toxicology 32(6):1299-1309.
  5. Chang YW, Singh KP (2019) Long-term exposure to arsenic induces fibrogenic changes in human kidney epithelial cells potentially through epigenetic mechanism. Journal of Cellular Physiology 234(4):4713-4725.
  6. Ponnusamy L, Mahalingaiah PK, Chang YW, and Singh KP (2018) Reversal of epigenetic aberrations associated with acquisition of doxorubicin resistance restores drug sensitivity in breast cancer cells. European J Pharmaceutical Sciences 123: 56-69.
  7. Ponnusamy L, Mahalingaiah PK, Singh KP (2017) Treatment schedule and estrogen receptor-status influence acquisition of doxorubicin resistance in breast cancer cells. European Journal of Pharmaceutical Sciences 104:424-433.
  8. Mahalingaiah PK, Ponnusamy L, Singh KP (2017) Oxidative stress-induced epigenetic changes associated with malignant transformation of human kidney epithelial cells. Oncotarget 8(7):11127-11143.
  9. Ponnusamy L, Mahalingaiah PK, and Singh KP (2016) Chronic oxidative stress increases resistance to doxorubicin-induced cytotoxicity in renal carcinoma cells potentially through epigenetic mechanism. Molecular Pharmacology 89(1):27 - 41.
  10. Tyagi T, Treas J, Mahalingaiah PK, and Singh KP (2015) Potentiation of growth inhibition and epigenetic modulation by combination of green tea polyphenol and 5-aza-2’ deoxycytidine in human breast cancer cells.  Submitted to Breast Cancer Research and Treatment 149(3):655-68.
  11. Mahalingaiah PK, Ponnusamy L, and Singh KP (2015) Chronic oxidative stress causes malignant transformation, along with acquisition of stem cell characteristics, and epithelial to mesenchymal transition in human renal epithelial cells. Journal of Cellular Physiology 230:1916-28.
  12. Okoh V, Garba N, Penney R, Das J, Deoraj A, Singh KP, Sarkar S, Felty Q, Yoo C, Jackson R, Roy D(2015)Redox signaling to nuclear regulatory proteins by reactive oxygen species contributes to estrogen-induced growth of breast cancer cells. British J Cancer 112:1687- 702.
  13. Treas J, Tyagi T, Singh KP (2013) Chronic exposure to arsenic, estrogen, and their combination causes increased growth and transformation in human prostate epithelial cells potentially by hypermethylation-mediated silencing of MLH1. The Prostate 73(15):1660 -1672.
  14. Singh KP, Treas J, Tyagi T, Gao W (2012) DNA demthylation by 5-aza-2-deoxycytidine treatment abrogates 17 beta-estradiol-induced cell growth and restores expression of DNA repair genes in human breast cancer cells. Cancer Letters 316 (1):62-69.
  15. Singh KP, Kumari R, Treas J, DuMond JW (2011) Chronic exposure to arsenic causes increased cell survival, DNA damage and increased expression of mitochondrial transcription factor A (mtTFA) in human prostate epithelial cells. Chemical Research in Toxicology 24: 340-349.
  16. Singh KP, Kumari R, and DuMond JW (2010) Simulated microgravity –induced epigenetic changes in human lymphocytes. Journal of Cellular Biochemistry 111:123-129.
  17. Singh KP, Kumari R, Pavey C, Jackson D, DuMond JW (2009) Long duration exposure to cadmium leads to increased cell survival, decreased DNA repair capacity, and genomic instability in mouse testicular Leydig cells. Cancer Letters 279:84-92.
  18. Singh KP, and Roy D (2008) Allelic loss and mutations in a new ETRG-1 gene are early events in DES-induced renal carcinogenesis in Syrian hamster.  GENE 408:18-26.
  19. Singh KP, and Roy D (2006) SKCG-1: a new candidate growth regulatory gene in human sporadic Wilm’s tumors. British J Cancer 94:1524-1532.
  20. Felty Q, Singh KP, and Roy D (2005) Estrogen-induced G1/S transition of G0 –arrested estrogen-dependent breast cancer cells is regulated by mitochondrial oxidant signaling. Oncogene 24: 4883-4893.

 

 

 

 

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The Department of Environmental Toxicology (ENTX) is the academic home for the core faculty of The Institute of Environmental and Human Health (TIEHH) and the Institute for Forensic Science (IFS) at Texas Tech University. TIEHH and IFS provide faculty and graduate students opportunities for multidisciplinary research and scholarly engagement related to environmental, forensic and human health sciences.

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